FAM NHS ester, 6-isomer，羧基荧光染料
Fluorescein (FAM) is a bright fluorophore that has found an extremely wide application in life science research. The fluorophore is hydrophilic and its derivatives possess good solubility in aqueous buffers.
Fluorescein is compatible with a wide variety of fluorescent instrumentation - most instruments are equipped with FAM channel filters.
This product contains the pure 6-isomer of fluorescein.
General properties：
Appearance:
yellow crystalline solid
Mass spec M+ increment:
358.0
Molecular weight:
473.39
Molecular formula:
C25NO9H15
IUPAC name:
(2,5-dioxopyrrolidin-1-yl) 3',6'-dihydroxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-carboxylate
Solubility:
good in DMF, DMSO
Quality control:
NMR 1H, HPLC-MS (95%)
Storage conditions:
Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
FAM NHS ester, 6-isomer，羧基荧光染料
Spectral properties：
Excitation maximum, nm:
494
ε, L⋅mol−1⋅cm−1:
75000
Emission maximum, nm:
520
Fluorescence quantum yield:
0.9
CF260:
0.20
CF280:
0.17